Web14 Feb 2024 · The full-length sequence of PvGF14c or PvFT were inserted at the C- and N-terminus of CaMV35S-GFP vector, forming PvGF14c-GFP or PvFT-GFP fusion protein, transient expression. The transient expression of the PvGF14-GFP or PvFT-GFP fusions in onion epidermal cells was made by the particle bombardment method ( Wang et al., 1988 ). Web27 Jul 2011 · In this study, we assessed the role of GF14e in rice disease resistance by suppressing its expression using an RNAi silencing approach. GF14e -silenced transgenic …
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Web600 ml Plastic Gravity Feed Paint Cup for EUROHE, EURHV and EUROVT Gravity Feed Spray Guns (GF14C) by Astro Pneumatic Tool®. Get your auto body repairs done quickly and efficiently with this paint spray product. Easy to use by pros and weekend garage junkies alike, it will come in handy if you need to do a restoration job on your vehicle to ... WebGF14c, GF14e, and GF14f) are induced by biotic and abiotic stress responses that interact with several stress-response-related proteins (Chen et al. 2006a, b; Cooper et al. 2003; Donald et al. 2006). Overexpression of Ara-bidopsis 14-3-3-k enhances drought tolerance in transgenic cotton plants (Yan et al. 2004). These studies indicate that
WebThe subcellular localization of FTL10 and GF14c, as well as BiFC analysis characterizing the interaction of FTL10 and GF14c, were carried out according to (Lin et al., 2024). For subcellular localization studies of FTL10 and GF14c, the coding regions of both FTL10 and GF14c were amplified with primers (Table S1) and fused with EGFP ... WebOsSCL7 can interact with GF14c, a 14‐3‐3 protein, and loss‐of‐function GF14c leads to enhanced susceptibility to M. oryzae. Additionally, OsSCL7 protein levels were reduced in …
Web1 Mar 2009 · GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in ... Web31 Mar 2024 · In this study, four 14-3-3 isoforms (GF14b, GF14c, GF14e, GF14f) exhibiting prominent expression profiles in rice roots, were selected for further investigation. Through a pull-down assay using four 14-3-3 isoforms in rice roots, 87 client proteins were identified that are involved in metabolism, protein synthesis and trafficking, energy metabolism, cell …
Web21 Aug 2007 · Twenty-seven of these clones contained cDNA fragments of members of the 14-3-3 gene family. They represented all eight isoforms of the rice 14-3-3 genes, namely GF14a, GF14b, GF14c, GF14d, GF14e, GF14f, GF14g, and GF14h. We selected GF14c, which appeared most frequently in the yeast two-hybrid screen (eight clones) for further studies.
Web15 Nov 2024 · GF14c, Hd3a, The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a, In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c gunfight in portsmouthWeb3 Nov 2024 · These genes are involved in different regulatory pathways, including G protein signaling pathway, ubiquitin-proteasome degradation pathway, mitogen-activated protein … bowne of atlantaWebGF14c, GF14f~OsGF14f, The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress, At least four rice GF14 genes, GF14b, GF14c, GF14e and Gf14f were differentially regulated in the interactions of rice-Magnaporthe grisea and … gunfight instant crushWebWelcome to Casino World! Play FREE social casino games! Slots, bingo, poker, blackjack, solitaire and so much more! WIN BIG and party with your friends! gunfight instant stopWeb18 Jan 2013 · To construct the GF14c RNAi vector, a 430-bp DNA fragment containing 69 bp of the 5′-untranslated region and 361 bp of the 5′-end coding region of GF14c was amplified using the GF14c-Ri forward primer (GF14c-F5) and GF14c-Ri reverse primer (GF14c-R5). bowne meaningWeb27 Jul 2011 · In rice ( Oryza sativa ), the GF14e gene, which encodes a 14-3-3 protein, is induced during effector-triggered immunity (ETI) associated with pathogens such as Xanthomonas oryzae pv. oryzae ( Xoo ). To determine whether the GF14e gene plays a direct role in resistance to disease in rice, we suppressed its expression by RNAi silencing. gunfight jeansWeb1 Feb 2024 · OsSCL7 can interact with GF14c, a 14‐3‐3 protein, and loss‐of‐function GF14c leads to enhanced susceptibility to M. oryzae. Additionally, OsSCL7 protein levels were reduced in the gf14c... bowne of boston