Hygromycin b resistance cassette

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August undefined, 2024

Web21 apr. 2014 · The hygromycin B (Hyg R) resistance marker used in this study confers resistance by the activity of a specific a minoglycoside ph osphotransferase encoded by … Web29 mrt. 2024 · A new expression cassette (EC0) consisting of the fused 5′ and 3′ intergenic regions (IGRs) of the Eremothecium cymbalariae translational elongation factor 1α (EcTEF1) gene was evaluated through expression of the bacterial hygromycin B phosphotransferase (hph) resistance gene in the common baker’s yeast …

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Webresistance cassette against hygromycin B with PGK Promoter. Use. Synthetic Biology Tags. Expression. Mutation. Promoter. Availability. Academic Institutions and Nonprofits only Enlarge pSH231-EF1-GFP-HYGRO. Plasmid #115144. Purpose. Safe harbor site 231 knock-in vector with GFP ... WebExpression plasmid with hygromycin resistance gene. 20 µg . pseth-mcs. TDS MSDS +-$535.00 Add to favorite. pSELECT-hygro-LacZ Unit size Cat. code Docs Qty ... the two expression cassettes are oriented in convergent directions ... Hygromycin B Gold . Selective antibiotic for the hph gene ... blackcraftcult sofa

Hygromycin B and Apramycin Antibiotic Resistance Cassettes for …

WebHygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980s. Mechanism of … WebHygromycin B. 41,42 Hygromycin B (Figure 8), produced by S. hygroscopicus, exhibits activities against both prokaryotic and eukaryotic cells. From: Comprehensive Medicinal … Web1 okt. 1999 · Two plasmids are constructed that can be used for cloning as templates for PCR- -based gene disruption, mutagenesis and the construction of DNA chromosome translocation cassettes, and are the first vectors that confer resistance to ampicillin, kanamycin and hygromycin B in bacteria, and to geneticin and hygein in … galway ireland province

Development of a yeast heterologous expression cassette based …

Hygromycin Resistance Cassette TaKaRa Bioz

WebArticle Snippet: The hygromycin B resistance cassette was amplified through PCR from the pCB1004 vector (Carroll et al ., ) using the Gibson assembly primer couple GibFor … WebCpD14 knockout mutants were obtained through double homologous recombination with a construct carrying a hygromycin B resistance cassette interrupting the gene sequence. Around 1 kb upstream and downstream sequences of the CpD14 gene were used to induce homologous recombination. galway ireland shopping areaWeb1 dec. 2003 · Therefore, the aim of this work was to design a more efficient gene knockout method for use in this yeast. To simplify the use of multiple selection markers, we have developed disruption cassettes containing the homologous ylURA3 and ylLEU2 genes, and the heterologous marker hph conferring resistance to hygromycin-B (Hyg) into the … black craft cult sweater

"WebHygromycin B resistance as dominant selectable marker in yeast. Saccharomyces cerevisiae is normally sensitive to the drug hygromycin B; a hygromycin B concentration … " - Hygromycin b resistance cassette

Hygromycin b resistance cassette

Hygromycin Resistance Cassette TaKaRa Bioz

WebCan anyone share a sequence of hygromycin resistance gene or any other antibiotic resistance gene (except mentioned above) that can be … Web26 jan. 2024 · Primary transformants were crossed with the color spore mutant fus1-1 (S23442) and single-spore isolates (ΔSmlon2) with hygromycin resistance were selected . The absence of the Smlon2 gene and the integration of the hph -cassette at the desired locus was verified using primer pairs lon2-ko-v5f/tC1_0 (1400 bp), h3_0/lon2-ko-v3r …

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WebTransformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers … Web24 dec. 2024 · Hygromycin B- and nourseothricin-resistant transformants with red ascospores were isolated, analyzed by PCR for correct integration of the deletion cassette, and crossed to wildtype to dispose of the NHEJ-deficiency and to recycle the nat1 marker.

Web13 jan. 2024 · All mutants showed similar mCherry expression (Fig. 4A and B) and stable integration of the construct was verified by Southern Blot, detecting the hygromycin resistance cassette (Fig. 4C). Due to the linearization with SmaI of the construct prior to transformation and the digestion of gDNA with EcoRV, bands with sizes of more than … Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding …

Web2 feb. 2024 · The cassette includes the FRT site (red), promoter site for hygromycin (green), and the hygromycin resistance gene (blue). (DOCX) History 2024-02-02 - First … WebVersatile co-expression of graft-protective proteins using 2A-linked cassettes . × Close Log In. Log in with Facebook Log in with Google. or. Email. Password. Remember me on this computer. or reset password. Enter the email address you signed up with and we'll email ...

WebHygR 345 .. 1373 = 1029 bp 342 amino acids = 38.1 kDa Product: aminoglycoside phosphotransferase from E. coli confers resistance to hygromycin HygR 345 .. 1373 = …

Web1 jan. 2010 · Gene-specific knockout cassettes should be designed to have 1-1.3 kb of gene-specific 5′ and 3′ flanks and a selectable marker (the hygromycin B phosphotransferase gene, hph, in our case). To ensure appropriate expression of the selectable marker, we use the A. nidulans promoter trpC ( 13 ). galway ireland to cork irelandWeb24 dec. 2024 · For gene deletion, we provide five different donor vectors for selection marker cassettes. These include standard cassettes for hygromycin B, nourseothricin and … galway ireland rental cottagesWeb1 dec. 2010 · Complementation of mutants was performed using hygromycin B resistance, utilizing the pYM70 plasmid (54) and generating a cassette with the HYG resistance gene at the tail end of the PCR product (52). blackcraft cult shower curtain black craft cult sleeveless hoodieWeb29 sep. 2024 · PDA plates supplemented with 20 μg/mL of hygromycin B resulted in complete growth inhibition of Lophiotrema sp. F6932 wild-type strain, an indication that hygromycin B resistance gene would be suited for used as a selectable marker for Lophiotrema sp. F6932 knockouts mutants (Supplementary Figure S4). Protoplast … black craft cult shortsWebCochliobolus heterostrophus was transformed with a plasmid (pH1S) containing a bacterial gene (hygB), which confers resistance to the antibiotic hygromycin B when under control of an 838-bp fragment of promoter 1 from C. heterostrophus. The plasmid integrated at either homologous (52% single copy, 33% tandemly repeated copies) or ectopic (4% … black craft cult storeWeb2 feb. 2024 · Sequence of hygromycin resistance cassette for mutant generation. The cassette includes the FRT site (red), promoter site for hygromycin (green), and the hygromycin resistance gene (blue). (DOCX) Browse. Search. Explore more content. ppat.1009291.s004. docx (20.59 kB) File info Download file. galway ireland to belfast ireland