Hieff clone plus multi one step cloning kit

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August undefined, 2024

WebYour search returned 43 Assay Kit across 1 supplier. Showing 30 of 43 products. 1; 2 > >> Sort By. Select All. Select up to 5 products from below to compare or request more information. Request Info for all products Compare. Viral RNA Isolation Kit Yeasen Biotechnology Co ... WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, …

Hieff CloneTM一步法同源重组快速定向克隆试剂盒 - 产品 ...

WebThe Hieff Clone™ Universal One Step Cloning Kit ensures efficient single- and multi-fragment cloning. A-B: Recombinant transformation plates. C: Electropherogram of … Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China). small tick symbol in word

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Web为了减少扩增突变的引入，建议使用高保真聚合酶进行扩增（Hieff Canace® High-Fidelity DNA Polymerase，Cat No. 10135ES）。 PCR扩增结束后，取少量产物进行琼脂糖凝胶 … WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … highway to heaven nct歌词

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http://www.bioon.com.cn/news/showarticle.asp?newsid=68004 WebBGC0001040: rapamycin biosynthetic gene cluster from Streptomyces rapamycinicus NRRL 5491. small thyroid nodules symptomsWebusing Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co. Ltd.) and the vectors were transformed into EscherichiacoliDH5 cells.Sequenceswereconﬁrmedby DNA sequencing. The plasmids were extracted by Plasmid Maxi Preparation Kit or Plasmid Maxiprep Kit (Bey-otime, Shanghai, China), and subsequently transfected into highway to heaven part 2

"Web17 de set. de 2024 · On one side, DRAM1 interacted ... BioID2 plasmid, respectively. 3FLAG-DRAM1 and GCaMP6 or DRAM1 and BioID2-FLAG were homologously recombined using the Hieff Clone® Plus Multi One Step Cloning Kit ... " - Hieff clone plus multi one step cloning kit

Hieff clone plus multi one step cloning kit

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Web13 de abr. de 2024 · 高保真聚合酶（Hieff Canace Plus High-Fidelity DNA Polymerase，货号 10153ES60）一步法克隆试剂盒（Hieff Clone ® Plus One Step Cloning Kit 一步 … http://yeasen.com/assets/upload/file/20240817130924833.pdf

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Web产品描述. Hieff Clone TM Plus Multi One Step Cloning Kit 是一款简便、快速、高效的 DNA 定向克隆产品，该试剂盒可以将 PCR 产物定向克隆至任何载体的任何位点，可高效克隆 50 bp-10 kb 片段。将载体线性化，并在插入片段正、反向 PCR 引物 5’ 端引入 15-25 bp 的线性化载体末端同源序列，使得插入片段 PCR 产物 ... WebDescription. The GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized ...

Web24 de abr. de 2024 · In summary, the multiple genes transformation increased the SF content in broccoli more than single gene introductions. The aim of this research was to improve sulforaphane content by introducing FMOGS–OX2, ... (Hieff Clone Plus Multi One Step Cloning Kit, Yeasen, shanghai, China). WebThe Hieff Clone™ Universal One Step Cloning Kit should be stored at -25℃~ -15°C for 1 year. Figures. Figure 1 Multi-segment linkage: performance comparison with competitive …

WebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。 … Web27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS …

WebGenBuilder™ Cloning Kit: GenBuilder™ Plus Cloning Kit: 1: Unique need fulfilled by the kit: Multi-gene cloning for up to 6 fragments: Multi gene cloning for up to 12 fragments: 2: Cost per reaction: $7.90: $9.80: 3: Number of fragments that can be cloned into a linearized vector: 6: 12: 4: Cloning efficiency (insert was cloned into the ...

http://yeasen.com/assets/upload/file/20240907084815442.pdf highway to heaven one winged angelWeb9 de dez. de 2024 · HR donor plasmids were constructed using the Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, China). The G622A HR donor was constructed by ligating four fragments (a left arm, ... highway to heaven on lifetimeWebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. highway to heaven s1e1WebHieff Clone® Plus Multi One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb … highway to heaven pilotWebItem Hieff Clone® Plus Multi One Step Cloning Kit; Company Yeasen Biotechnology Co., Ltd; Catalog Number 10912; This product is no longer available on Biocompare. … small ticket equipment leasing companiesWeb20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … highway to heaven parents dayWebBasic Steps of Cloning. There are many approaches available for cloning DNA, most of which follow this basic workflow: Isolation of target DNA - typically referred to as an … small tic tacs