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Associate Dir/Medical Director - Dermatology in …
WebJul 22, 2024 · To determine the migration of iMSC-sEV into the brain, iMSC-sEV were stained with DiR (Thermo Fisher, USA) according to the protocol as previously described with small modification. Briefly, sEV were incubated with DiR fluorescent dye under room temperature for 15 min, followed by ultracentrifugation at 100,000 g in PBS to get rid of … Webcontaining 20 µM DiR (Thermo Fisher Scientific) or 80 µM 3'-OMe-DiR for 30 minutes at 37 °C (prepared from 2.5 mM DMSO stock). The cells were washed twice with PBS, mounted on a slide glass with PBS, and observed with a fluorescence microscope (BX61; Olympus America, Melville, NY, USA). deta of science
Michael A. Dirr - About Horticulture - UGA
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