WebFeb 18, 2024 · A novel d-allulose 3-epimerase gene (daeM) has been identified from the metagenomic resource of a hot-water reservoir.The enzyme epimerizes d-fructose into d-allulose, a functional sugar of rare abundance in nature. The metagenomic DNA fragment was cloned and expressed in Escherichia coli The purified recombinant protein (DaeM) … Weblocation: 153, residue: K. PRK09722. PRK09722 (1-227): allulose-6-phosphate 3-epimerase. TIM superfamily. cl21457 (1-227): A large family of domains similar to triose phosphate isomerase (TIM) which, in general, share an eight beta/alpha closed barrel structure. click domain graphic to toggle highlight of corresponding residues in 3D viewer.
Metabolically Engineered Escherichia coli for Conversion of D …
WebAug 17, 2024 · In addition, Agm4 shows sequence similarity to d-allulose-6-phosphate (A6P) 3-epimerase (Fig. S3). Thus, we hypothesized that Agm4 catalyzes the epimerization of fructose 6-phosphate to form d ... WebMar 1, 2006 · We now report functional and structural studies of d-allulose 6-phosphate 3-epimerase (ALSE) from Escherichia coli K-12 that catalyzes the equilibration of the hexulose 6-phosphates d-allulose 6 ... highest rated show on wb
Bioproduction of D-allulose: Properties, applications, purification ...
http://www.globalauthorid.com/WebPortal/AuthorView?wd=be7111df-cfab-4992-b109-b47a7bbdfd6e WebAn epimerase protein of fructose-6-phosphate which has at least 70% of amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1 and converts fructose-6-phosphate to allulose-6-phosphate. The epimerase protein according to claim 1, wherein the enzyme is encoded by a nucleotide sequence having at least 80% of nucleotide … WebPutative D-allulose-6-phosphate 3-epimerase UniProtKB InterPro STRING Interactive Modelling. 221 aa; Sequence (Fasta) It is possible new templates exist for this target since these models were created. Rebuild Models. Available Structures . 1 SWISS-MODEL model. Template Oligo-state QMEANDisCo Range Ligands Trg-Tpl Seq id (%) ... highest rated shows amazon prime 2019