WebBL21(DE3)感受态细胞由特殊工艺制作,pUC19质粒检测转化效率达10 7 cfu/μg DNA。 操作方法 1.BL21(DE3)感受态细胞从-80℃拿出,迅速插入冰中,5分钟后待菌块融化,加入目的DNA(质粒或连接产物)并用手拨打EP管底轻轻混匀(避免用枪吸打),冰中静置25分钟。 WebDescription Intact Genomics chemically competent BL21 (DE3) E. coli cells are suitable for transformation and routine protein expression. Specifications Competent cell type: Chemically competent Derivative of: BL21 (DE3) Species: E. coli Format: Tubes Transformation efficiency: ≥1.0 x 10 9 cfu/µg pUC19 DNA Blue/white screening: Yes
DH10Bac Chemically Competent Cell 大肠杆菌化学感受态细胞 - 上 …
WebSingle-Use BL21(DE3)pLysS Competent Cells, >106cfu/µg 1ml (20 × 50µl) L1195 Storage Conditions: Always store Competent Cells at –70°C. Thaw on ice when ready for use. Do not refreeze thawed, unused aliquots. Competent cells, supplied in 200µl aliquots, are provided with 3ng of competent cells control DNA for use as a positive WebBL21, or C43 competent cells spread on IPTG plates to induce protein expression. C41 BL21 C43 Strain BL21(DE3) C41(DE3) C43(DE3) ... (DE)pLysS, and C43(DE3) pLysS, as chemically competent cells, as indicated. Expression Recovery Medium is also available separately. 24-reaction kits are multiples of the 12 reaction kit, 2 × 12 reactions. Which ... real estate practice by dearborn login
BL21(DE3) Competent Cells - Novagen - Sigma-Aldrich
WebDH10B Chemically Competent Cell大肠杆菌化学感受态细胞. 10×100μl. MF2315-1000UL. XL10-Gold Chemically Competent Cell大肠杆菌化学感受态细胞 . 10×100μl. MF2331-1000UL. BL21 (DE3) Competent Cell大肠杆菌感受态细胞. 10×100μl. MF2332-1000UL. BL21 (DE3) pLysS Competent Cell大肠杆菌感受态细胞. 10×100μl ... WebBL21 (DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site. BL21 (DE3)pLysS is lysogenic for λ-DE3, which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter. how to tell pregnancy news to husband